Unique sequence specificity of topoisomerase II DNA cleavage stimulation and DNA binding mode of streptonigrin.
نویسندگان
چکیده
منابع مشابه
Induction of mammalian DNA topoisomerase II dependent DNA cleavage by antitumor antibiotic streptonigrin.
Streptonigrin, a nonintercalative antitumor antibiotic, induced mammalian topoisomerase II dependent DNA cleavage in vitro. The cleavage activity of streptonigrin was comparable to that of demethylepipodophyllotoxin ethylidene-beta-D-glucoside at a low concentration (less than or equal to 10 microM) but one-third lower at a higher concentration (greater than 250 microM). Exposure of a reaction ...
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Using the P4 unknotting assay, DNA topoisomerase I1 has been purified from several mammalian cells. Similar to prokaryotic DNA gyrase, mammalian DNA topoisomerase I1 can cleave double-stranded DNA and be trapped as a covalent protein-DNA complex. This cleavage reaction requires protein denaturant treatment of the topoisomerse 11-DNA complex and is reversible with respect to salt and temperature...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1994
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)31490-4